Apollo benefits

     Apollo® ultrafiltration devices are used for purification, concentration or desalting of proteins and retroviral vectors, offering maximal benefit for processing of very dilute hydrophobic macrosolutes which may require use of surfactants to solubilize.  

  The high-performance ultrafiltration of  Apollo Concentrators (US patents 6,269,957, 6,357,601) is the result of accelerated conical focusing of retained macrosolute combined with improved use of regenerated cellulose membrane.  The conical membrane active filtration area extends below the level of the permeate ports, which supports a high filtration rate as the port height deadstop volume is approached. When the sample meniscus reaches the level of the permeate ports, hydrostatic pressure equalizes across the active membrane below the ports and filtration stops.

  The steep angle of the cone assures continuous removal of denser retained protein as it slides

down into the recovery cup formed at the tip of the cone. This depolarization improves

performance compared to designs with membrane placed at shallower angles to the centrifugal

field.

  The larger active membrane area reduces protein exposure to sticky plastic surfaces.

Apollo Concentrators deliver quantitative recovery of micrograms of retained protein (>90%).  

They focus all the retentate into a single drop with minimal film loss on bounding surfaces. This facilitates direct pipette recovery without the need for an inverted spin recovery step.

   The Apollo conical design accepts a larger sample volume for a given tube size than other devices which constrict the membrane in a separate thin lower chamber. 

  Apollo Concentrators feature an airtight-sealed internal vent, which improves bio-safety by eliminating aerosol generation, and by eliminating need for hand contact with wetted surfaces (no invert spin required). 

   Apollo 7 mL provides up to four times more salt/buffer exchange in a single spin (1000x concentration factor).